Abstract Background Traditional Map based Cloning approaches. used for the identification of desirable alleles. are extremely labour intensive and years can elapse between the mutagenesis and the detection of the polymorphism. High throughput sequencing based Mapping-by-sequencing approach requires an ordered genome assembly and cannot be used with fragmented. https://safeersappliancers.shop/product-category/zanussi-zggn755k-built-in-74cm-5-burners-gas-hob-black-lpg-convertible/